Fluorescence-in-situ-hybridization (FISH) of fungal pathogens in a spiked bronchoalveolar lavage sample (BAL). From left to right: Visualization of double stranded DNA in fungi and host cells with DAPI (blue), hybridization with unspecific eukaryotic probe (green), - with specific probe Mucorales (Magenta), - with specific probe for Aspergillus (orange), overlay of all channels documents the presence of diverse fungi