Standard preparation of SARS new Coronavirus for diagnostic purposes.

Description

The samples you obtained contain cell culture supernatant of VeroE6 infected with the new Coronavirus causing the "severe acute respiratory syndrome" (SARS). The samples were thoroughly analysed for infectivity after inactivation by heat and gamma irradiation. According to the inactivation procedures used, we assure you that we provide you with safe and non-biohazard material which can be handled under normal laboratory conditions. The virus preparation was diluted in human plasma before aliquoting and freeze drying.

-- The sample must be resolved by adding 100 μl bi.dest. water before use. --

Analysis of the samples

The samples contain a mean of 9.4 x 10⁶ genome equivalents per ml (geq/ml) analysed by Christian Drosten, Bernhard-Nocht-Institut, Hamburg, Germany.

In electron microscopy analysis performed by Hans Gelderblom, Robert Koch-Institut, Berlin, Germany we could demonstrate the presence of Coronavirus particles in the samples.

The analysis of the infectivity titre of the cell culture supernatant before inactivation is presently on the way and can be given later on request.

Acknowledgement:

The virus isolate was provided by Prof. Dr. Doerr and his colleagues from the University of Frankfurt, Germany and is described in more detail by:

Drosten C., Günther S., Preiser W., van der Werf S., Brodt H-R., Becker S., et al. (2003): Identification of a novel coronavirus in patients with severe acute respiratory syndrome. N Engl. J. Med. published at www.nejm.org on 10 April 2003 (10.1056/NEJMoa030747).
http://content.nejm.org/cgi/reprint/NEJMoa030747v2.pdf

The sample preparation was performed at the Robert Koch-Institut in Berlin, Germany under the grant from the European Community DG SANCO and was provided by the European Network for Diagnostics of "Imported" Viral Diseases (ENIVD).

We would kindly like to ask you to acknowledge these contributions in publications mentioning these standard: SARS standard was kindly provided by Matthias Niedrig, Robert Koch-Institut, Berlin

For further requests, please contact: Prof. Dr. Matthias Niedrig,
Robert Koch-Institut, Berlin, Germany
Phone: ++49-1888-754-2370, Fax: ++49-1888-754-2625

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